Composite

Part:BBa_K136022:Design

Designed by: Kok-Phen YAN   Group: iGEM08_Paris   (2008-10-25)


Weak Promoter - ECFP tripart (+LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ECFP (backward insert) cloned into the plasmid J61002 containing the weak promotor.


Source

Assembly of different parts from the registry.

References